ASSOCIATION OF RADIOACTIVE CHROMIUM WITH VARIOUS COMPONENTS OF HEMOGLOBIN

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Blood (U.S.)

OSTI ID:4755897

Malcolm, D; Ranney, H M; Jacobs, A S

Normal hemoglobin labeled with Cr/sup 51/ by incubation of whole blood with Na/sub 2/Cr/sup 51/O/sub 4/, or by incubation of hemolysates with either Na/ sub 2/Cr/sup 51/O/sub 4/ or Cr/sup 51/Cl/sub 3/ and most of the C r/sup 51/ activity was associated with the electrophoretically rapid hemoglobin A/sub 3/. The formation of a Cr/sup 51/-hemoglobin complex with an isoelectric point lower than that of hemoglobin was thought to be the most likely explanation of this finding. The radioactivity of Cr/sup 51/-labeled hemolysates containing hemoglobin S, C, and A was found to be associated with the more rapid electrophoretic components. This supports the hypothesis of a lower isoelectric point of a Cr-hemoglobin complex. The association of Cr/sup 51/ activity with polypeptide chains of hemoglobin was also studied. Cr/sup 51/ was found in both the naturally occurring BETA chain component (hemoglobin H) and in the BETA chain component. This supports the hypothesis that BETA chains of hemoglobin have a greater affinity for Cr than do alpha chains. (P.C.H.)

• Research Organization: Albert Einstein Coll., New York and Bronx Municipal Hospital, New York
• NSA Number: NSA-17-015593
• OSTI ID: 4755897
• Journal Information: Blood (U.S.), Journal Name: Blood (U.S.) Vol. Vol: 21; ISSN BLOOA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
CHROMIUM 51
COMPLEXES
CONFIGURATION
ELECTROPHORESIS
HEMOGLOBIN
LABELLED COMPOUNDS
MOLECULES
PREPARATION
QUANTITATIVE ANALYSIS
RADIOACTIVITY