Acetyltransferase and human hemoglobin acetylation

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5333028

Kasten-Jolly, J; Qiu, C C; Abraham, E C

A minor component of human fetal hemoglobin (Hb F) is acetylated at the amino-terminus of the ..gamma..-globin chains. A similar minor component of Hb F is formed during translation of cord blood mRNA in the rabbit reticulocyte lysate system. The acetylation appeared to be enzymatic. This system contains an acetyltransferase capable of acetylating histones and hemoglobins. The enzyme, partially purified by histone-Sepharose affinity chromatography was capable of incorporating labeled acetyl- group from 1-(/sup 14/C-acetyl)-CoA into both human Hb F/sub 0/ and HB A/sub 0/, but at a lower rate than for histones. Characterization of the labeled products indicated that the ..cap alpha..-chains of both hemoglobins were being acetylated presumably at a lysyl-residue, but in the case of Hb F/sub 0/ the amino-terminus of the ..gamma..-globin chains was acetylated as well. While histone-Sepharose bound more than 95% of the enzyme, Sepharose linked Hb F/sub 0/, ..gamma..-globin chains, and Hb Bart's bound 14, 5, and 12% of the activity, respectively. Enzyme bound to these resins was not any more active on the hemoglobins than was the enzyme bound to the histone-Sepharose. The histone-Sepharose was also used to detect the enzyme in human cord blood red cells separated by dextran 40 density gradient centrifugation. Activity was found mostly in the young cells, and was directly related to the number of reticulocytes present in any one fraction.

• Research Organization: Medical College of Georgia, Augusta
• OSTI ID: 5333028
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLATION
ACYLATION
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CENTRIFUGATION
CHEMICAL REACTIONS
CHROMATOGRAPHY
ENZYMES
FETUSES
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PRIMATES
PROTEINS
PURIFICATION
RABBITS
RNA
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES