Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Human red cell acetyltransferase

Journal Article · · Life Sci.; (United States)
Acetyltransferase was isolated by histone-Sepharose affinity chromatography from human cord blood red cells. The enzyme was detected only in very young red cells. The semipurified enzyme and (/sup 14/C)acetyl-CoA were used to acetylate isolated Hb F tetramer and ..cap alpha.. and ..gamma.. subunits. The in vitro acetylated products were characterized by globin chain separation by CM-cellulose chromatography and tryptic peptide analysis by reverse-phase HPLC. Acetylation of both the ..gamma..-chains and the ..cap alpha..-chains could occur within the Hb F tetramer. Acetylation also could take place on intact subunits. It appears that some Hb F/sub Ic/ could be formed in the cells by utilizing Hb F or free ..gamma..-chains as acetylation substrate.
Research Organization:
Medical College of Georgia, Augusta (USA)
OSTI ID:
6994921
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 42:26; ISSN LIFSA
Country of Publication:
United States
Language:
English