Identification of a hypoxic population of bone marrow cells
Journal Article
·
· Int. J. Radiat. Oncol., Biol. Phys.; (United States)
A technique using collagenase has been devised to release and separate, with reproducibility, hematopoietic cells (HC) from various microenvironments of mouse femurs. HC were assayed by an in vitro gel culture technique used traditionally to score granulocyte-macrophage precursor cells (CFU-C). CFU-C which resided in the medullary cavity and endosteal regions were sensitive to ionizing radiation and resistant to misonidazole (MISO) cytotoxicity. CFU-C which resided within the compact bone were resistant to ionizing radiation and sensitive to the cytotoxic action of MISO. These results suggest that HC which reside in the bone are hypoxic and retain clonogenic potential. When animals were exposed to various treatments with MISO followed by myelotoxic doses of cyclophosphamide (CTX) or total body irradiation (TBI), the LD/sub 50/ of both agents was significantly reduced. This result suggests that a hypoxic component of HC could be important in the regenerative process within the marrow after such myelotoxic trauma.
- Research Organization:
- Univ. of Alberta, Canada
- OSTI ID:
- 5502552
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 9:2; ISSN IOBPD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550603 -- Medicine-- External Radiation in Therapy-- (1980-)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANOXIA
ANTINEOPLASTIC DRUGS
AZOLES
BONE MARROW CELLS
CFU
CHEMOTHERAPY
CONNECTIVE TISSUE CELLS
DRUGS
ENDOXAN
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IMMUNOSUPPRESSIVE DRUGS
IN VITRO
IONIZING RADIATIONS
MEDICINE
MISONIDAZOLE
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATIONS
RADIOLOGY
RADIOSENSITIVITY
RADIOSENSITIZERS
RADIOTHERAPY
SOMATIC CELLS
THERAPY
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANOXIA
ANTINEOPLASTIC DRUGS
AZOLES
BONE MARROW CELLS
CFU
CHEMOTHERAPY
CONNECTIVE TISSUE CELLS
DRUGS
ENDOXAN
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IMMUNOSUPPRESSIVE DRUGS
IN VITRO
IONIZING RADIATIONS
MEDICINE
MISONIDAZOLE
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATIONS
RADIOLOGY
RADIOSENSITIVITY
RADIOSENSITIZERS
RADIOTHERAPY
SOMATIC CELLS
THERAPY