Protective, restorative, and therapeutic properties of recombinant colony-stimulating factors

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Blood; (USA)

OSTI ID:5513231

Talmadge, J E; Tribble, H; Pennington, R; Bowersox, O; Schneider, M A; Castelli, P; Black, P L; Abe, F ^[1]

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1. National Cancer Institute-Frederick Cancer Research Facility, MD (USA)

Pretreatment of mice with recombinant murine (rM) colony-stimulating factor-granulocyte-macrophage (CSF-gm) or recombinant human (rH) CSF-g provides partial protection from the lethal effects of ionizing radiation or the alkylating agent cyclophosphamide (CTX). In addition, these agents can significantly prolong survival if administered following lethal doses of irradiation or CTX. To induce protective activity, cytokines were injected 20 hours before lethal irradiation or CTX administration. To accelerate recovery from lethal irradiation, the cytokines must be administered shortly following irradiation, and the induction of maximal levels of activity is dependent on chronic administration. In contrast, because of their longer half-lives, accelerated recovery from alkylating agents requires a delay of at least 24 to 48 hours to allow complete clearance of CTX before administration of a CSF. Studies quantitating peripheral blood leukocytes and bone marrow cellularity as well as colony-forming units per culture (CFU-C) frequency and CFU-C per femur revealed a significant correlation between these parameters and the ability to survive lethal irradiation.

• OSTI ID: 5513231
• Journal Information: Blood; (USA), Journal Name: Blood; (USA) Vol. 73:8; ISSN 0006-4971; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL TISSUES
ANIMALS
BLOOD FORMATION
BODY
BONE MARROW
COLONY FORMATION
DISEASES
DRUGS
ENDOXAN
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
IMMUNOSUPPRESSIVE DRUGS
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
IRRADIATION
LETHAL IRRADIATION
LEUKOPENIA
MAMMALS
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOSENSITIVITY EFFECTS
RODENTS
TISSUES
VERTEBRATES