Therapeutic administration of recombinant human granulocyte colony-stimulating factor accelerates hemopoietic regeneration and enhances survival in a murine model of radiation-induced myelosuppression

Journal Article · Thu Mar 01 04:00:00 EST 1990 · International Journal of Cell Cloning; (USA)

DOI:https://doi.org/10.1002/stem.5530080204· OSTI ID:6853390

Patchen, M L; MacVittie, T J; Solberg, B D; Souza, L M ^[1]

---

1. Armed Forces Radiobiology Research Institute, Bethesda, MD (USA)

The primary cause of death after radiation exposure is infection resulting from myelosuppression. Because granulocytes play a critical role in host defense against infection and because granulocyte proliferation and differentiation are enhanced by granulocyte colony-stimulating factor (G-CSF), this agent was evaluated for the ability to accelerate hemopoietic regeneration and to enhance survival in irradiated mice. C3H/HeN mice were irradiated and G-CSF (2.5 micrograms/day, s.c.) or saline was administered on days 3-12, 1-12 or 0-12 post-irradiation. Bone marrow, splenic and peripheral blood cellularity, and bone marrow and splenic granulocyte-macrophage progenitor cell recoveries were evaluated in mice exposed to 6.5 Gy. Mice exposed to 8 Gy were evaluated for multipotent hemopoietic stem cell recovery (using endogenous spleen colony-forming units) and enhanced survival. Results demonstrated that therapeutic G-CSF (1) accelerates hemopoietic regeneration after radiation-induced myelosuppression, (2) enhances survival after potentially lethal irradiation and (3) is most effective when initiated 1 h following exposure.

• OSTI ID: 6853390
• Journal Information: International Journal of Cell Cloning; (USA), Journal Name: International Journal of Cell Cloning; (USA) Vol. 8:2; ISSN 0737-1454; ISSN IJCCE
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
DISEASES
EXTERNAL IRRADIATION
GROWTH FACTORS
HEMIC DISEASES
INJURIES
IRRADIATION
LEUKOCYTES
MAMMALS
MATERIALS
MICE
MITOGENS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATION INJURIES
RADIOSENSITIVITY EFFECTS
RODENTS
SURVIVAL TIME
SYMPTOMS
VERTEBRATES
WHOLE-BODY IRRADIATION