Survival enhancement and hemopoietic regeneration following radiation exposure: Therapeutic approach using glucan and granulocyte colony-stimulating factor

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Experimental Hematology (Lawrence, Kansas); (USA)

OSTI ID:6509177

Patchen, M L; MacVittie, T J; Solberg, B D; Souza, L M ^[1]

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1. Armed Forces Radiobiology Research Institute, Bethesda, MD (USA)

C3H/HeN female mice were exposed to whole-body cobalt-60 radiation and administered soluble glucan (5 mg i.v. at 1 h following exposure), recombinant human granulocyte colony-stimulating factor (G-CSF; 2.5 micrograms/day s.c., days 3-12 following exposure), or both agents. Treatments were evaluated for their ability to enhance hemopoietic regeneration, and to increase survival after radiation-induced myelosuppression. Both glucan and G-CSF enhanced hemopoietic regeneration alone; however, greater effects were observed in mice receiving both agents. For example, on day 17 following a sublethal 6.5-Gy radiation exposure, mice treated with saline, G-CSF, glucan, or both agents, respectively, exhibited 36%, 65%, 50%, and 78% of normal bone marrow cellularity, and 84%, 175%, 152%, and 212% of normal splenic cellularity. At this same time, granulocyte-macrophage colony-forming cell (GM-CFC) values in saline, G-CSF, glucan, or combination-treated mice, respectively, were 9%, 46%, 26%, and 57% of normal bone marrow values, and 57%, 937%, 364%, and 1477% of normal splenic values. Endogenous spleen colony formation was also increased in all treatment groups, with combination-treated mice exhibiting the greatest effects. Likewise, although both glucan and G-CSF alone enhanced survival following an 8-Gy radiation exposure, greatest survival was observed in mice treated with both agents. These studies suggest that glucan, a macrophage activator, can synergize with G-CSF to further accelerate hemopoietic regeneration and increase survival following radiation-induced myelosuppression.

• OSTI ID: 6509177
• Journal Information: Experimental Hematology (Lawrence, Kansas); (USA), Journal Name: Experimental Hematology (Lawrence, Kansas); (USA) Vol. 18:9; ISSN EXHMA; ISSN 0301-472X
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BONE MARROW
COBALT 60
COBALT ISOTOPES
COLONY FORMATION
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MACROPHAGES
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
MITOGENS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PROTEINS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
STEM CELLS
SUBLETHAL IRRADIATION
SURVIVAL TIME
SYNERGISM
TISSUES
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOT