Selenium dependent glutathione-peroxidase (GSH-Px) activity in the retina of preterm human infants
GSH-Px activity was determined in the retina of 15 preterm human neonates with gestational ages of 17-28 weeks and birth weights of 120 to 960 g. GSH-Px activity was measured using the coupled assay. The infants survived from 0.5 to 9 hours after parturition. The retinas were removed within 3 hours of autopsy. Through electronmicroscopy, there was verification that the entire retina was removed and no contamination of other eye tissues occurred. After removal, the retinas were immediately dissolved in phosphate buffered pH 7.0 saline for assay of GSH-Px activity. The mean GSH-Px activity was 19.44 +/- 6.44 with a range of 11.1 to 32.8 units NAPH/sub 2/ oxidized/min/g protein. There was a negative correlation between birth weight and GSH-Px activity (r = -0.86) and between week of gestation and GSH-Px activity (r = -0.91). The neonatal retina GSH-Px activity was 2 to 15 times higher than found in adult retinas. Thus, this research demonstrates that selenium dependent GSH-Px activity is elevated in the preterm neonate's retina which indicates that retina GSH-Px activity may be an important antioxidation system in the premature neonate.
- Research Organization:
- Auburn Univ., AL
- OSTI ID:
- 5492632
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PEROXIDASES
ENZYME ACTIVITY
SELENIUM
BIOLOGICAL EFFECTS
INFANTS
RETINA
SURVIVAL TIME
AGE GROUPS
BODY
BODY AREAS
CHILDREN
ELEMENTS
ENZYMES
EYES
FACE
HEAD
ORGANS
OXIDOREDUCTASES
SEMIMETALS
SENSE ORGANS
560306* - Chemicals Metabolism & Toxicology- Man- (-1987)