Effect of endothelial cell denudation on fatty acid metabolism by the rabbit aorta

Conference · Mon Feb 26 04:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5488213

Takasaki, I; Cohen, R A; Chobanian, A V; Brecher, P ^[1]

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1. Boston Univ. School of Medicine, MA (United States)

The regulatory role of aortic endothelial cells in fatty acid uptake and metabolism by vascular tissue was studied in vitro using aortic strips from New Zealand white rabbits. Endothelium was left intact or removed either by scraping with forceps or with a cotton swab. Removal of endothelium was assessed morphologically by silver staining and pharmacologically by endothelium-dependent vasorelaxation. Aortic strips were incubated with {sup 14}C-oleate (OA) or {sup 14}C-arachidonate (AA) and oxidation and esterification was measured. Denudation by forceps caused a 50% decrease in oxidation and esterification into phospholipid (PL) and triglyceride (TG) for both OA and AA. In contrast, removal by cotton swab caused a smaller but significant reduction in oxidation (OA; 26%, AA; 24%), and esterification into PL was reduced by only 8% (OA) or 17% (AA) and TG incorporation was not affected for either fatty acid. In the presence or absence of endothelium, esterification of AA into PL was significantly higher than that of OA, with a differing distribution among PL classes between OA and AA. The studies suggest endothelium has a role in fatty acid uptake and metabolism, and that fatty acid oxidation may be a sensitive index of vascular injury.

• OSTI ID: 5488213
• Report Number(s): CONF-9004153--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MARKERS
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
ENDOTHELIUM
ESTERS
LIPIDS
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXIDATION
PHOSPHOLIPIDS
RABBITS
TISSUES
VERTEBRATES