Transendothelial transport and metabolism of adenosine and inosine in the intact rat aorta

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Circ. Res.; (United States)

DOI:https://doi.org/10.1161/01.RES.64.6.1147· OSTI ID:5879012

Kroll, K; Kelm, M K; Buerrig, K F.S.; Schrader, J

This study was aimed at defining the role of vascular endothelium in the transport and metabolism of adenosine. For this purpose, endothelium-intact and endothelium-denuded isolated rat aortas, perfused at constant flow (2 ml/min), were prelabeled with 3H-adenosine or 3H-inosine for 10 minutes at concentrations of 0.012-100 microM. Sequestration of adenosine by endothelium was determined from radioactivity recovered during selective endothelial cell removal with deoxycholic acid (0.75% for 15 seconds). In the physiological concentration range of adenosine (0.012-1 microM), fractional sequestration by endothelium was 90-92% of the total adenosine incorporation by the aorta. Endothelial sequestration of inosine at 0.1 microM was 85%. At 100 microM adenosine or inosine, fractional sequestration by aortic endothelium was 33% and 39%, respectively. Analysis of the specific radioactivity of adenine nucleotides extracted from prelabeled aortas indicated that most of the adenosine was incorporated into endothelial adenine nucleotides. Incorporation of inosine into endothelial ATP was approximately 15% that of adenosine. Inhibition of aortic adenosine deaminase with erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) did not influence sequestration of 0.1 microM adenosine, but resulted in a 49% reduction of total endothelial incorporation at 100 microM adenosine. Transfer of radioactive purines from the endothelium to underlying smooth muscle after prelabeling was equivalent to only 1%/hr of total endothelial radioactivity.

• Research Organization: Univ. of Duesseldorf (Germany, F.R.)
• OSTI ID: 5879012
• Journal Information: Circ. Res.; (United States), Journal Name: Circ. Res.; (United States) Vol. 64:6; ISSN CIRUA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENINES
ADENOSINE
AMINES
ANIMAL TISSUES
ANIMALS
ANTIMETABOLITES
AORTA
AROMATICS
ARTERIES
ATP
AZAARENES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
DRUGS
ENDOTHELIUM
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROLASES
INOSINE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PERFUSED TISSUES
PURINES
RATS
RIBOSIDES
RODENTS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES