Influence of dietary fat on metabolism of (14-/sup 14/C)erucic acid in the perfused rat liver. Distribution of metabolites in lipid classes

Journal Article · Sun Jun 01 04:00:00 EDT 1986 · Lipids; (United States)

OSTI ID:5018770

Holmer, G; Ronneberg, R

Two groups of rats were fed diets containing 20% by weight of either partially hydrogenated marine oil supplemented with sunflower seed oil (PHMO) or palm oil (PO) for 8 wk. Using a liver perfusion system, the effect of dietary long chain monoenoic fatty acids on the uptake and metabolism of (14-/sup 14/C)erucic acid was studied. The perfusion times were 15 and 60 min, respectively. The two groups showed equal ability for erucic acid uptake in the liver but differed in the channeling of the fatty acids into various metabolic pathways. A higher metabolic turnover of 22:1 in the PHMO livers relative to the PO livers was demonstrated by an increased recovery of total (/sup 14/C)labeling in the triglyceride (TG) and phospholipid (PL) fractions, already evident after 15 min of perfusion. The chain-shortening capacity was highest in the PHMO group, reflected by a higher (/sup 14/C)18:1 incorporation in both TG and PL, and increasing from 15 to 60 min of perfusion. The amount of (/sup 14/C)18:1 found in PL and TG after 60 min of perfusion of livers from rats fed PO corresponded to that shown for the PHMO group after 15 min. The PL demonstrated a discrimination against 22:1 compared to TG, and, when available, 18:1 was highly preferred for PL-synthesis. The total fatty acid distribution in the TG, as determined by gas liquid chromatography (GLC), reflected the composition of the dietary fats. In the total liver PL, 22:1 and 20:1 were present in negligible amounts, although the PHMO diet contained 12-13% of both 22:1 and 20:1. In the free fatty acid fraction (FFA), the major part of the radioactivity (approximately 80%) was (14-/sup 14/C)erucic acid, and only small amounts of (/sup 14/C)18:1 (less than 2%) were present, even after 60 min of perfusion. The shortened-chain 18:1 was readily removed from the FFA pool and preferentially used for lipid esterification.

• Research Organization: Technical Univ. of Denmark, Lyngby
• OSTI ID: 5018770
• Journal Information: Lipids; (United States), Journal Name: Lipids; (United States) Vol. 6; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
ESTERS
FATS
GLANDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIVER
MAMMALS
METABOLISM
METABOLITES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
TRACER TECHNIQUES
TRIGLYCERIDES
UPTAKE
VERTEBRATES