Lipid composition and metabolism in embryos of Brassica napus

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5850809

Sparace, S A ^[1]; Pomroy, M K ^[2]

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1. McGill Univ., Ste Anne de Bellevue, Quebec (Canada)
2. Agriculture Canada, Ottawa, Ontario (Canada)

Seven and 14-day old microspore-derived developing embryos of the low-erucate Brassica napus L. (cv. Topas) were analyzed for their acyl lipid composition and capacity to incorporate ({sup 14}C)acetate into lipid. The most significant changes in the lipid compositions of these ages of embryos are (1) increased total lipid from 2 to 5% of fresh weight; (2) increased proportion of TAG from 31 to 74%, and shifts in the fatty acid composition of TAG from 25 to 50% 18:1; 28 to 23% 18:2; and 24 to 13% 18:3. Lipids of 7-day embryos also consist of primarily 8% DAG, 2% MG, 12% FFA, 10% DGDG, 15% PA and approximately 5% each of PC, PE and PG. The levels of these lipids generally decrease as the embryos mature and accumulate TAG. ({sup 14}C)Acetate is incorporated into all lipids and fatty acids except 18:2 or 18:3. As much as 39, 59 and 34% of the fatty acid radioactivity of Mg was recovered in 20:0, 22:0 and 24:0, respectively.

• OSTI ID: 5850809
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
BRASSICA
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
FOOD
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METABOLISM
ORGANIC COMPOUNDS
PLANTS
TRACER TECHNIQUES
VEGETABLES