Storage lipid biosynthesis in microspore-derived Brassica napus embryos

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6650286

Taylor, D C; Underhill, E W; Weber, N ^[1]; Pomeroy, M K ^[2]; Edwards, L ^[3]

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1. Plant Biotechnology Institute, Saskatoon (Canada)
2. Plant Research Centre, Ottawa, Ontario (Canada)
3. Biotechnica Inc., Calgary (Canada)

Erucic acid, a fatty acid which is confined to the neutral lipids in developing seed cotyledons or rape, was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. cv Reston microspore-derived embryo culture system. Accumulation and changes in acyl composition of TAGs during embryogenesis strongly paralleled that observed during seed development. Homogenates of 29-day cultured embryos were examined for the ability to incorporate erucoyl moieties into storage lipids. In the presence of {sup 14}C erucoyl CoA and various acceptors, including glycerol-3-phosphate (G3P), {sup 14}C erucic acid was rapidly incorporated into the TAG fraction. However, in contrast to studies with {sup 14}C oleoyl CoA, there was no measurable radioactivity in any Kennedy Pathway intermediates or within membrane lipid components. Analysis of the radiolabelled TAG species suggested that erucoyl moieties were incorporated into the sn-3 position by a highly active diacylglyercol acyltransferase.

• OSTI ID: 6650286
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MARKERS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BRASSICA
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
EMBRYOS
ENZYMES
FOOD
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ONTOGENESIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
VEGETABLES