Glucose and fatty acid metabolism in normal and diabetic rabbit cerebral microvessels

Journal Article · Fri May 01 04:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:5723015

Hingorani, V; Brecher, P

Rabbit cerebral microvessels were used to study fatty acid metabolism and its utilization relative to glucose. Microvessels were incubated with either (6-/sup 14/C)glucose or (1-/sup 14/C)oleic acid and the incorporation of radioactivity into /sup 14/CO/sub 2/, lactate, triglyceride, cholesterol ester, and phospholipid was determined. The inclusion of 5.5 mM glucose in the incubation mixture reduced oleate oxidation by 50% and increased esterification into both phospholipid and triglyceride. Glucose oxidation to CO/sub 2/ was reduced by oleate addition, whereas lactate production was unaffected. 2'-Tetradecylglycidic acid, an inhibitor of carnitine acyltransferase I, blocked oleic acid oxidation in the presence and absence of glucose. It did not effect fatty acid esterification when glucose was absent and eliminated the inhibition of oleate on glucose oxidation. Glucose oxidation to /sup 14/CO/sub 2/ was markedly suppressed in microvessels from alloxan-treated diabetic rabbits but lactate formation was unchanged. Fatty acid oxidation to CO/sub 2/ and incorporation into triglyceride, phospholipid, and cholesterol ester remained unchanged in the diabetic state. The experiments show that both fatty acid and glucose can be used as a fuel source by the cerebral microvessels, and the interactions found between fatty acid and glucose metabolism are similar to the fatty acid-glucose cycle, described previously.

• Research Organization: Boston Univ. School of Medicine, MA
• OSTI ID: 5723015
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:5; ISSN AJPHA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BIOLOGICAL PATHWAYS
BLOOD VESSELS
BODY
BRAIN
CAPILLARIES
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CEREBRUM
CHALCOGENIDES
CHEMICAL REACTIONS
DIABETES MELLITUS
DISEASES
ENDOCRINE DISEASES
ESTERS
GLUCOSE
HEXOSES
HYDROXY ACIDS
LABELLED COMPOUNDS
LACTIC ACID
LIPIDS
MAMMALS
METABOLIC DISEASES
METABOLISM
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NERVOUS SYSTEM
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXIDATION
OXIDES
OXYGEN COMPOUNDS
PHOSPHOLIPIDS
RABBITS
SACCHARIDES
TRIGLYCERIDES
VERTEBRATES