Induction of sister chromatid exchanges by direct and indirect chemical agents in a human teratoma cell line
- Florida International Univ., Miami, FL (USA). Dept. of Biological Sciences
In the present work, we have extended the characterization of the P3 cell line, derived from a human epithelial teratocarcinoma, by studying the induction of sister chromatid exchanges (SCEs) by direct and indirect carcinogens. Several direct-acting carcinogens produce a dose-dependent increase in SCEs. Most notably, N-methyl-N{prime}-nitro-N-nitrosoguanidine and 7{beta}, 8{alpha}-dihydroxy-9 {alpha},10{alpha}-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene produce increases in SCEs at dosages comparable to those used to induce mutations at the hypoxanthine-guanine phosphoribosyl transferase locus. The indirect carcinogens elicit SCEs only when the P3 cells are cocultured with cells capable of metabolizing the indirect carcinogens to the active form. Human breast carcinoma (BJ-015) and rat hepatoma (RL12) cells are equally efficient in activating polycyclic aromatic hydrocarbons to the active form. This cell-mediated induction of SCEs is obtained when P3 cells are incubated with live, x-irradiated, or UV-irradiated BJ or RL cells. This P3 cell line is thus equally suitable to study the induction of mutations or the induction of SCEs with direct and indirect carcinogens. 35 refs., 3 tabs.
- Research Organization:
- Argonne National Lab., IL (USA)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 5481738
- Report Number(s):
- ANL/PPRNT-89-191; ON: DE90003004
- Country of Publication:
- United States
- Language:
- English
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