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Radiosterilization of rat liver microsome containing postmitochondrial supernatant for mutation assays

Journal Article · · Environ. Mutagen.; (United States)
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Gamma-irradiation was effectively employed to sterilize rat liver postmitochondrial supernatant (PMS), which is required for the metabolic activation of soots and soot-derived polycyclic aromatic hydrocarbons to mutagens. When a known number of Bacillus subtilis spores were added to the PMS and gamma-irradiated at -80 degrees C, a 2-Mrad dose resulted in a 7.5 log kill of the spores. A dose of 3 Mrads was selected as a sufficient effective sterilizing dose and had no significant effect upon the ability of gamma-irradiated PMS to metabolically activate diesel soot and two diesel soot components, benzo(a)pyrene and fluoranthene to mutagens in a Salmonella typhimurium 8-azaguanine resistance forward mutation assay. Three Mrads of gamma-irradiation also had no effect upon the ability of PMS to activate benzo(a)pyrene to a mutagen for the human lymphoblasts. However, gamma-irradiation did reduce the ability of PMS to activate dimethylnitrosamine to a mutagen for S typhimurium.

Research Organization:
Genetic Toxicology Research Group, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge
OSTI ID:
5478057
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 3:5; ISSN ENMUD
Country of Publication:
United States
Language:
English

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Related Subjects

550700 -- Microbiology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAGUANINE
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BENZOPYRENE
BODY
CELL CONSTITUENTS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
DRUGS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GLANDS
HYDROCARBONS
IONIZING RADIATIONS
LIVER
MAMMALS
METABOLIC ACTIVATION
MICROORGANISMS
MICROSOMES
MUTAGEN SCREENING
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
POLYCYCLIC AROMATIC HYDROCARBONS
RADIATIONS
RADIOSTERILIZATION
RATS
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SOOT
SPORES
STERILIZATION
VERTEBRATES