Mutation of human cells by kerosene soot

Journal Article · Wed Aug 01 00:00:00 EDT 1979 · J. Natl. Cancer Inst.; (United States)

OSTI ID:5260332

Skopek, T R ^[1]; Liber, H L; Kaden, D A; Hites, R A; Thilly, W G

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1. Massachusetts Inst. of Tech., Cambridge, MA

The polycyclic aromatic hydrocarbon fraction of a kerosene soot induced forward mutation in human diploid lymphoblasts when coincubated with coincubated with Sprague-Dawley rat liver postmitochondrial supematant. Two components of the kerosene soot extract, benzo(a)pyrene (BP) and cyclopenta(cd)pyrene (CP), were also tested. BP was not mutagenic at the concentration found in the soot extract, although it was active at higher concentrations. The amount of CP present could account for approximately 8% of the total mutation observed with the soot. The results were compared to data obtained previously in a similar mutation assay in Salmonella typhimurium. the protocol described permits the facile assay of mutation at the hgprt locus in human lymphoblasts; such mutation is induced by compounds or complex mixtures requiring mixed-function oxygenase activity for metabolism to genetically active derivatives.

• OSTI ID: 5260332
• Journal Information: J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 63:2; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020600 -- Petroleum-- Health & Safety
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
ANIMAL CELLS
ANIMALS
AROMATICS
BACTERIA
BENZOPYRENE
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
ENZYMES
FUELS
HYDROCARBONS
KEROSENE
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
METABOLIC ACTIVATION
MICROORGANISMS
MIXED-FUNCTION OXIDASE SYSTEMS
MUTAGENESIS
MUTAGENS
MUTATIONS
ORGANIC COMPOUNDS
OXIDASES
OXIDOREDUCTASES
PETROLEUM PRODUCTS
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
PRIMATES
SALMONELLA
SALMONELLA TYPHIMURIUM
SOMATIC CELLS
SOOT
VERTEBRATES