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Radiosterilization of rat liver microsome containing postmitochondrial supernatant for mutation assays. [/sup 60/Co]

Journal Article · · Environ. Mutagen.; (United States)
; ; ;

Gamma-irradiation was effectively employed to sterilize rat liver postmitochondrial supernant (PMS), which is required for the metabolic activation of soots and soot-derived polycyclic aromatic hydrocarbons to mutagens. When a known number of Bacillus subtilis spores were added to the PMS and gamma-irradiated at -80/sup 0/C, a 2-Mrad dose resulted in a 7.5 log kill of the spores. A dose of 3 Mrads was selected as a sufficient effective sterilizing dose and had no significant effect upon the ability of gamma-irradiated PMS to metabolically activate diesel soot and two diesel soot components, benzo(a)pyrene and fluoranthene to mutagens in a Salmonella typhimurium 8-azaguanine resistance forward mutation assay. Three Mrads of gamma-irradiation also had no effect upon the ability of PMS to activate benzo(a)pyrene to a mutagen for the human lymphoblasts. However, gamma-irradiation did reduce the ability of PMS to activate dimethylnitrosamine to a mutagen for S typhimurium.

Research Organization:
Massachusetts Inst. of Tech., Cambridge
DOE Contract Number:
AC02-77EV04267
OSTI ID:
5440889
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 3; ISSN ENMUD
Country of Publication:
United States
Language:
English

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Related Subjects

550700 -- Microbiology
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BACTERIAL SPORES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL CONSTITUENTS
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GLANDS
HYDROCARBONS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LIVER
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
METABOLIC ACTIVATION
MICROORGANISMS
MICROSOMES
MINUTES LIVING RADIOISOTOPES
MUTAGEN SCREENING
NITROSAMINES
NITROSO COMPOUNDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
POLYCYCLIC AROMATIC HYDROCARBONS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSTERILIZATION
RATS
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SOMATIC CELLS
SOOT
SPORES
STERILIZATION
SURVIVAL CURVES
VERTEBRATES
YEARS LIVING RADIOISOTOPES