Changes in the nuclear protein kinase activities in the regenerating liver of partially irradiated rat

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577521· OSTI ID:5466789

Asami, K; Kobayashi, H; Fujiwara, A; Yasumasu, I ^[1]

---

1. National Institute of Radiological Sciences, Tokyo (Japan)

X rays (4.8 Gy) inhibit both DNA synthesis and phosphorylation of histone H1 in the regenerating liver of the rat. To determine the cause of the inhibition of histone H1 phosphorylation, changes in the nuclear protein kinase activities during the prereplicative phase of regeneration were measured. The cAMP-dependent protein kinase activity was low during regeneration, and the changes in the activity were not statistically significant. The cAMP-independent protein kinase activity increased at 15 h, decreased at 18 h, and increased again at 24 h after partial hepatectomy. X irradiation prior to partial hepatectomy did not inhibit the increase at 15 h, but it did inhibit the increase at 24 h. The activity was not inhibited by isoquinolinesulfonamide inhibitors such as H-7, and it was activated by a commercial preparation of an inhibitor protein of the cAMP-dependent kinase. It was also inhibited by quercetin. The possibility that the radiation-sensitive nuclear protein kinase is a nuclear cAMP-independent protein kinase specific for histone H1 is considered.

• OSTI ID: 5466789
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 119:3; ISSN 0033-7587; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BODY
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
GLANDS
HEPATECTOMY
HISTONES
INHIBITION
IONIZING RADIATIONS
LIVER
MAMMALS
MEDICINE
NUCLEIC ACID REPLICATION
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RATS
RECOVERY
RODENTS
SURGERY
TRANSFERASES
VERTEBRATES
X RADIATION