EFFECTS OF WHOLE-BODY IRRADIATION AND OF AET ON THE ACTIVITY OF THYMIDINE KINASES IN REGENERATING LIVER

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Arch. Intern. Physiol. Biochem.

OSTI ID:4679059

Goutier, R; Baugnet-Mahieu, L; Semal, M

Rats were injected with 100 mg/kg AET (aminoethylisothiuronium) dihydrochloride 10 min before exposure to a whole-body dose of 1000 r, partially hepatectomized 1.5 to 2 hr after irradiation, and sacrificed 12 to 48 hr later. In homogenates of the regenerating liver, the activity of thymidine kinases was measured and expressed as the proportion of thymidine converted to mono-, di-, and triphosphates (TMP, TDP, and TTP). The kinases of nonirradiated liver removed 24 hr after hepatectomy converted 47% of the thymidine to TMP, 4.5% to TDP, and 2.1% to TTP, giving a total conversion of 54% to the nucleotides. At this time the activity was highest; at 36 and 48 hr of regeneration the phosphorylation of thymidine reached 50 and 36%, respectively. In irradiated liver at 24 hr after hepatectomy the kinase activity was very low, as previously shown, with only 3 to 4% phosphorylation of thymidine. But this reflects only a delay in the appearance of the enzyme, since at 36 hr a value of 23% is reached and at 48 hr the value is 47%, which is higher than in nonirradiated liver. Injection of AET did not prevent the depletion of kinase activity caused by irradiation; in fact, the percentage of thymidine phosphorylated was lower when AET was given. Injection of AET in nonirradiated rats produced almost 50% inhibition of kinase activities. (TCO)

• Research Organization: Centre d'Etude de l'Energie Nucleaire, Mol, Belg.
• NSA Number: NSA-17-025045
• OSTI ID: 4679059
• Journal Information: Arch. Intern. Physiol. Biochem., Journal Name: Arch. Intern. Physiol. Biochem. Vol. Vol: 71
• Country of Publication: Country unknown/Code not available
• Language: French

Related Subjects

AET
AMINES
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BODY
ENZYMES
ETHYL RADICALS
LIVER
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION DOSES
RADIATION EFFECTS
RATS
REGENERATION
SURGERY
THYMIDINE
UREA