Human interleukin 1. beta. (IL-1. beta. ), a more powerful inducer of bone demineralization than interleukin 1. cap alpha. IL-1. cap alpha. ), parathyroid hormone (PTH) or prostaglandin E/sub 2/ (PGE/sub 2/) in vitro

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5463384

Chin, R C; Hodges, Y C; Allison, A C

Effects of human IL-1..cap alpha.. and IL-1..beta.., prepared by recombinant DNA technology on cultures of rat fetal long bones, prelabelled with /sup 45/Ca were studied. IL-1..beta.. was found to be the most powerful inducer of bone calcium loss so far known. Maximal activity (2.5 times the control rate of calcium loss) was induced by IL-1..beta.. at concentrations between 1 x 10/sup -10/ M to 6 x 10/sup -12/ M. With IL-1..cap alpha.. maximal activity (1.5 times the control rate of calcium loss) was obtained at 6 x 10/sup -10/ M. With bovine PTH (1-34) maximal activity (1.8 times the control rate of calcium loss) was obtained at 1 x 10/sup -8/ M. With PGE/sub 2/ maximal activity (1.6 times the control rate of calcium loss) was obtained at 1 x 10/sup -7/ M. The calcium loss induced by IL-1..beta.. was inhibited in the presence of 1 x 10/sup -7/ M indomethacin, 5 x 10/sup -5/ M naproxen or ketorolac, or 5 x 10/sup -6/ M cyclohexamide. These findings suggest that protein synthesis and prostaglandin formation are required to mediate bone demineralization induced by IL-1..beta...

• Research Organization: Syntex Research, Palo Alto, CA
• OSTI ID: 5463384
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CALCIUM 45
CALCIUM ISOTOPES
CATTLE
CELL CULTURES
DAYS LIVING RADIOISOTOPES
DEMINERALIZATION
DOMESTIC ANIMALS
EVEN-ODD NUCLEI
GROWTH FACTORS
HORMONES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LYMPHOKINES
MAMMALS
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARATHORMONE
PEPTIDE HORMONES
PROSTAGLANDINS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
RUMINANTS
SEPARATION PROCESSES
SKELETON
TRACER TECHNIQUES
VERTEBRATES