Interleukin-1 beta (IL-1 beta) induces thrombocytosis in mice: Possible implication of IL-6
- Fukushima Medical College (Japan)
We administered recombinant human interleukin-1 beta (IL-1 beta), the common mediator of inflammation process, to C57B1/6 male mice (0.5 microgram, every 12 hours over five times) intraperitoneally and consequently induced a remarkable thrombocytosis. Day 1 was designated as the following day of the last injection in the morning. A significant thrombocytosis was observed on days 1 through 5 with a peak on day 2 (162 +/- 9 x 10(4)/mm3) compared with the control mice injected with heated IL-1 beta (101 +/- 11 x 10(4)/mm3). A striking increase in mean size of marrow megakaryocytes was noted on days 1 and 2. The incorporation of 75Se-selenomethionine into circulating platelets as a measure of platelet production was about 2.3 times higher in IL-1 beta-treated mice than in control mice. To determine which factor(s) is responsible for elicited thrombocytosis, the in vitro studies and bioassays for several hematopoietic factors were performed. IL-1 beta by itself did not stimulate megakaryocytopoiesis in vitro, suggesting that the thrombocytosis is attributed to other factor(s) via IL-1 beta stimulation. Serum colony-stimulating factor (CSF) activity after a single IL-1 beta (0.5 microgram) injection, monitored by colony assay with 10% tested serum, peaked at 3 hours. Formed colonies were mostly granulocyte (G) and granulocyte-macrophage (GM)-types, and studies using rabbit anti-mouse GM-CSF serum or using human marrow as target cells showed that the CSF activity of the tested serum consisted of, at least, GM-CSF and G-CSF. Addition of IL-3 concomitantly with the tested serum gave rise to a greater number of megakaryocytic colonies. Serum IL-3, monitored by IL-3-dependent cell line 32D clone 5, and erythropoietin activities were not detected at serum level in IL-1 beta-treated mice.
- OSTI ID:
- 6249514
- Journal Information:
- Blood (Journal of Hematology); (USA), Vol. 76:12; ISSN 0006-4971
- Country of Publication:
- United States
- Language:
- English
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BLOOD PLATELETS
BIOSYNTHESIS
LYMPHOKINES
BIOCHEMICAL REACTION KINETICS
THROMBOSIS
PATHOGENESIS
BONE MARROW
CEREBROSPINAL FLUID
COLONY FORMATION
ERYTHROPOIETIN
INTRAPERITONEAL INJECTION
METHIONINE
MICE
SELENIUM 75
STEM CELLS
TRACER TECHNIQUES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPOTROPIC FACTORS
MAMMALS
MATERIALS
MITOGENS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SELENIUM ISOTOPES
SOMATIC CELLS
SYNTHESIS
TISSUES
VASCULAR DISEASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
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