Comparative effects of thrombopoietin and interleukin-6 on murine megakaryocytopoiesis and platelet production

Journal Article · Fri Feb 15 04:00:00 EST 1991 · Blood (Journal of Hematology); (USA)

OSTI ID:6011073

McDonald, T P; Cottrell, M B; Swearingen, C J; Clift, R E ^[1]

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1. Univ. of Tennessee, Knoxville (USA)

A thrombocytopoiesis-stimulating factor (TSF or thrombopoietin) derived from human embryonic kidney (HEK) cells is known to increase platelet production and to increase the number of morphologically unrecognizable early megakaryocytes, ie, small acetylcholinesterase-positive (SAChE+) cells in mice. Other recent studies have concluded that interleukin-6 (IL-6) also stimulates murine megakaryocytopoiesis both in vitro and in vivo. Some workers have suggested that IL-6 is thrombopoietin. Therefore, the purpose of this study was to compare the effects of TSF and IL-6 on percent 35S incorporation into platelets, platelet sizes, and the percentages of SAChE+ cells in C3H mice, and to determine if they produce the same or different responses. The results showed that two or four injections of a partially purified TSF (total dose of 2 or 4 units (U) over a 1- or 2-day period) increased percent 35S incorporation into platelets (P less than .005) and platelet sizes (P less than .005) of both normal and rebound-thrombocytotic mice when compared with values from other mice treated with human serum albumin, the carrier protein for both TSF and IL-6. In eight separate experiments, it was shown that IL-6 (40,000 U, 4 micrograms), when given to rebound-thrombocytotic mice in four injections over a 2-day period, produced a small but significant (P less than .005) increase in percent 35S incorporation into platelets. Additional studies showed that negative results were obtained when similar high doses of IL-6 were administered in two doses over a 1-day period. TSF, but not IL-6, stimulated an increase in platelet sizes of normal mice (P less than .005 to 0.0005); however, IL-6 increased platelet sizes of rebound-thrombocytotic mice when given in two of four injections (P less than .05 to .0005).

• OSTI ID: 6011073
• Journal Information: Blood (Journal of Hematology); (USA), Journal Name: Blood (Journal of Hematology); (USA) Vol. 77:4; ISSN BLOOA; ISSN 0006-4971
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD COUNT
BLOOD FORMATION
BLOOD PLATELETS
BODY FLUIDS
CARBOXYLESTERASES
CHOLINESTERASE
COAGULANTS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
GROWTH FACTORS
HEMATOLOGIC AGENTS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LYMPHOKINES
MAMMALS
MATERIALS
MICE
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
THROMBOPOIESIS
TRACER TECHNIQUES
VERTEBRATES