Interleukin 1. cap alpha. and interleukin 1. beta. bind to the same receptor on T cells

Journal Article · Wed Jan 01 04:00:00 EST 1986 · J. Immunol.; (United States)

OSTI ID:5783789

Kilian, P L; Kaffka, K L; Stern, A S; Woehle, D; Benjamin, W R; Dechiara, T M; Gubler, U; Farrar, J J; Mizel, S B; Lomedico, P T

Pure, E. coli-derived recombinant murine interleukin 1..cap alpha.. (IL 1..cap alpha..) was labeled with /sup 125/I and used for receptor binding studies. The /sup 125/I-IL 1 binds to murine EL-4 thymoma cells in a specific and saturable manner. Scatchard plot analysis for binding studies carried out at 4/sup 0/C reveals a single type of high affinity binding site with an apparent dissociation constant of approximately 2.6 X 10/sup -10/ M and the presence of approximately 1200 binding sites per cell. Unlabeled recombinant murine IL 1 competes for /sup 125/I-IL 1 binding in a dose-dependent manner, whereas interferon-..cap alpha..A, interleukin 2 (IL 2), epidermal growth factor, and nerve growth factor have no effect. The /sup 125/I-IL 1 binding site is sensitive to trypsin, suggesting that it is localized on the cell surface. The authors have also examined the ability of purified recombinant human IL 1..cap alpha.. and IL 1..beta.. to compete for binding of the radiolabeled murine IL 1 to its receptor and to stimulate IL 2 production by EL-4 cells. They report here that both human IL 1 proteins are able to recognize the same binding site on mouse IL 1. In addition, murine as well as both human IL 1 proteins stimulate IL 2 production by EL-4 cells.

• Research Organization: Hoffmann-La Roche, Inc., Nutley, NJ
• OSTI ID: 5783789
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
REACTION KINETICS
SOMATIC CELLS
TRACER TECHNIQUES