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Properties of a specific interleukin 1 (IL 1) receptor on human Epstein Barr virus-transformed B lymphocytes. Identity of receptor for IL 1-. cap alpha. and IL 1-. beta

Journal Article · · J. Immunol.; (United States)
OSTI ID:5785625
; ; ; ;
The properties of specific human interleukin 1 (IL 1) receptors on human Epstein Barr virus-transformed B lymphocytes (EBV-B) were studied. Purified human IL 1-..beta.. from a myelomonocytic cell line (THP-1) was labeled with /sup 125/I. Among four EBV-B cell lines tested, a pre-B cell type (VDS-O) specifically bound the highest amount of /sup 125/I-IL 1-..beta... The binding of /sup 125/I-IL 1-..beta.. to VDS-O cells was inhibited by F(ab)'/sub 2/ fragments of anti-human IL 1 and recombinant human IL 1-..cap alpha.., as well as by unlabeled human IL 1-..beta.. but not by recombinant lymphotoxin, recombinant tumor necrosis factor, or phorbol myristic acid, suggesting that IL 1-..cap alpha.. and IL 1-..beta.. bind specifically to the same receptor. The m.w. of IL 1 receptor on human EBV-B cells was estimated to be 60,000 by both the chemical cross-linking method and high pressure liquid chromatography (HPLC). The isoelectric point of solubilized human IL 1 receptor was 7.3 on HPLC chromatofocusing. The evidence of existence of IL 1 receptor on human EBV-B cells additionally supports the hypothesis that IL 1 may be an autocrine signal for these cells.
Research Organization:
National Institutes of Health, Frederick, MD
OSTI ID:
5785625
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GEL PERMEATION CHROMATOGRAPHY
GLOBULINS
GROWTH FACTORS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MICROORGANISMS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
REACTION KINETICS
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
VIRUSES