Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Properties of an EBV-B cell line derived interleukin 1 (IL 1) receptor

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5392937
; ; ; ;
The properties of an human IL 1 receptor on a human EBV-B line were studied. Purified human IL 1-..beta.. produced by a myelomonocytic cell line (THP-1) was labeled with /sup 125/I by the Bolton-Hunter method without loss of biological activity. Among four EBV-B cell lines tested, a pre-B cell type (VDS-O) specifically bound the most /sup 125/I IL-..beta... Maximal binding was reached within 20 min at 4/sup 0/C. Scatchard analysis of the binding of /sup 125/I-IL 1-..beta.. to VDS-O cells yielded a Kd of 2.4-5.9 x 10/sup -00/ M with 110 to 220 binding (receptor) sites/cell. The binding of /sup 125/I-IL 1-..beta.. to VDS-O cells was inhibited by anti-human IL 1 antibody, natural and recombinant human IL 1-..cap alpha.. as well as IL 1-..beta.., but not by IFN-..cap alpha.., TNF, or LT, suggesting that IL 1-..cap alpha.. and IL 1-..beta.. specifically bind to the same receptor. The mw of the IL 1 receptor on human EBV-B cells was estimated to be 60 Kd both by a chemical crosslinking method and by HPLC gel filtration analysis of solubilized receptor extracted from membranes by a nonionic detergent (CHAPS). The pI of solubilized human IL 1 receptor was 7.3 by HPLC chromatofocusing. Data showing that VDS-O cells proliferate in response to exogenously added IL 1, express IL 1 receptors and also produce IL 1 all support the hypothesis that IL 1 may function as an autocrine signal for B lymphocytes.
Research Organization:
Lab. of Molecular Immunoregulation, Frederick, MD
OSTI ID:
5392937
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

Similar Records

Properties of a specific interleukin 1 (IL 1) receptor on human Epstein Barr virus-transformed B lymphocytes. Identity of receptor for IL 1-. cap alpha. and IL 1-. beta
Journal Article · Tue Dec 31 23:00:00 EST 1985 · J. Immunol.; (United States) · OSTI ID:5785625
Detection of high affinity receptor sites for IL 1. beta. on a human B lymphoblastoid line which fail to recognize IL 1. cap alpha
Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6851399
Receptors for B cell stimulatory factor 2. Quantitation, specificity, distribution, and regulation of their expression
Journal Article · Thu Oct 01 00:00:00 EDT 1987 · J. Exp. Med.; (United States) · OSTI ID:5842242

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CELL PROLIFERATION
CHEMICAL PROPERTIES
CHEMICAL REACTIONS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES