Phosphoinositide metabolism and adrenergic receptors in astrocytes

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5450358

Noble, E P; Ritchie, T; de Vellis, J

Agonist-induced phosphoinositide (PI) breakdown functions as a signal generating system. Diacylglycerol, one breakdown product of phosphotidylinositol-4,5-diphosphate hydrolysis, can stimulate protein kinase C, whereas inositol triphosphate, the other product, has been proposed to be a second messenger for Ca/sup + +/ mobilization. Using purified astrocyte cultures from neonatal rat brain, the effects of adrenergic agonists and antagonists at 10/sup -5/ M were measured on PI breakdown. Astrocytes grown in culture were prelabeled with (/sup 3/H)inositol, and basal (/sup 3/H) inositol phosphate (IP/sub 1/) accumulation was measured in the presence of Li/sup +/. Epinephrine > norepinephrine (NE) were the most active stimulants of IP/sub 1/ production. The ..cap alpha../sub 1/ adrenoreceptor blockers, phentolamine and phenoxybenzamine, added alone had no effect on IP/sub 1/ production was reduced below basal levels. Propranolol partially blocked the effects of NE. Clonidine and isoproterenol, separately added, reduced IP/sub 1/ below basal levels and when added together diminished IP/sub 1/ accumulation even further. The role of adrenergic stimulation in the production of c-AMP.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 5450358
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ADRENALINE
ALKALI METAL COMPOUNDS
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
BRAIN
CALCIUM
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATIONS
CELL CULTURES
CENTRAL NERVOUS SYSTEM
CHARGED PARTICLES
DRUGS
ELEMENTS
ENZYMES
HORMONES
INOSITOL
INOSITOLS
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LITHIUM COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
METABOLISM
METALS
MONOSACCHARIDES
NEONATES
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RATS
RECEPTORS
RODENTS
SACCHARIDES
SOMATIC CELLS
STEROID HORMONES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES