Effect of. cap alpha. /sub 1/-adrenergic stimulation on phosphoinositide metabolism and protein kinase C (PK-C) in rat cardiomyocytes

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5435771

Kaku, T; Lakatta, E; Filburn, C R

Alpha/sub 1/-adrenergic stimulation is known to enhance membrane phospholipid metabolism resulting in increases in inositol phosphates (IP's) and diacylglycerol (DAG). Cardiomyocytes prelabeled with /sup 3/H-myo-inositol were treated with norepinephrine (NE) for 1-15 min, acid extracted, and IP's separated by ion exchange chromatography. Addition of NE (10/sup -5/M) in the presence of propranolol (10/sup -5/ M) and LiCl (9 mM) enhanced the accumulation of IP's, linearly with time up to 15 min, and reached 7.3, and 1.5-fold at 15 min for IP/sub 1/, IP/sub 2/, and IP/sub 3/, respectively. KCl at 30 mM had no effect on accumulation of IP's, but augmented the effect of NE. PK-C activity was measured in both cytosol (S) and particulate (P) fractions of treated cells. NE alone had a negligible effect on membrane PK-C, while 30 mM KCl caused a small increase. However, pretreatment with KCl followed by NE produced a significant increase above that seen with KCl alone. Dioctanoylglycerol also stimulated membrane association of PK-C in these cells. These data suggest that ..cap alpha../sub 1/-adrenergic stimulation of membrane association of myocardial PK-C is mediated by DAG but may be dependent on membrane potential and/or the extent of Ca/sup 2 +/ loading.

• OSTI ID: 5435771
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ALCOHOLS
ALKALI METAL COMPOUNDS
ALKALINE EARTH METALS
ANIMAL CELLS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BODY
CALCIUM
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL MEMBRANES
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
DRUGS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GLYCEROL
HALIDES
HALOGEN COMPOUNDS
HEART
HORMONES
HYDROXY COMPOUNDS
INOSITOL
INOSITOLS
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANES
METABOLISM
METALS
MONOSACCHARIDES
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POTASSIUM CHLORIDES
POTASSIUM COMPOUNDS
SACCHARIDES
SEPARATION PROCESSES
STEROID HORMONES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS