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Inositol phospholipid hydrolysis in cultured astrocytes and oligodendrocytes

Journal Article · · Life Sci.; (United States)
; ; ; ;

Cultures of astrocytes and oligodendrocytes were prelabeled with /sup 3/H-inositol and the accumulation of /sup 3/H-inositol phosphates was determined following stimulation with a number of neuroactive substances. In astrocytes, norepinephrine (NE) produced the greatest stimulation with significant increase also observed with bradykinin. In oligodendrocytes, the greatest stimulation was produced by carbachol with significant increase also produced by bradykinin, histamine and NE. Carbachol was found to be ineffective in producing stimulation in astrocytes. The accumulation of /sup 3/H-inositol phosphates in astrocytes in response to NE was found to be dependent on the presence of Li/sup +/. The NE stimulation in astrocytes was dose-dependent and had an EC/sub 50/ of 1.2 ..mu..M. This stimulation was blocked by the low concentration of the ..cap alpha../sub 1/-adrenergic antagonist prazosin but not by the ..cap alpha../sub 2/-adrenergic antagonist yohimbine. The NE-stimulated accumulation of /sup 3/H-inositol phosphates in astrocytes was inhibited by the cyclic nucleotide phosphodiesterase inhibitor isobutylmethylxanthine as well as by the cAMP analog dibutyryl cAMP. 34 references, 4 figures, 4 tables.

Research Organization:
Univ. of California, Los Angeles
OSTI ID:
6500898
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 41:1; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CULTURES
DATA
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ESTERS
EXPERIMENTAL DATA
HORMONES
INFORMATION
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
METABOLISM
MONOSACCHARIDES
NERVE CELLS
NEUROREGULATORS
NORADRENALINE
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORIC ACID ESTERS
SACCHARIDES
SOMATIC CELLS
STEROID HORMONES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS