Aberrant activation and regulation of the oxidative burst in neutrophils with Mo1 glycoprotein deficiency

Journal Article · Tue Jul 15 04:00:00 EDT 1986 · J. Immunol.; (United States)

OSTI ID:5448418

Nauseef, W M; de Alarcon, P; Bale, J F; Clark, R A

Patients whose cells are deficient in the glycoproteins LFA-1, Mo1, and p150,95 have recurrent infections and pronounced abnormalities in neutrophil adherence, aggregation, chemotaxis, and phagocytosis. Activation and regulation of oxidative metabolism of Mo1-deficient neutrophils have been characterized. These cells failed to depolarize or to produce O/sub 2//sup -/ or H/sub 2/O/sub 2/ normally when stimulated by opsonized zymosan. The chemotactic peptide formyl methionyl-leucyl-phenylalanine depolarized Mo1-deficient neutrophils normally but caused supernormal production of O/sub 2//sup -/ and H/sub 2/O/sub 2/, a result of a prolonged burst in oxidative metabolism. Phorbol myristate acetate depolarized Mo1-deficient neutrophils at a nearly normal rate but evoked release of significantly less O/sub 2//sup -/ and H/sub 2/O/sub 2/ than from normal PMN. The aberrant activation and regulation of the oxidative burst in Mo1-deficient neutrohpils are considered in light of recently neutrophils are considered in light of recently emerging concepts in the cell biology of this process, and the possibility that these abnormalities reflect a defect in the cytoskeleton-membrane interaction is discussed.

• Research Organization: Univ. of Iowa, Iowa City
• OSTI ID: 5448418
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 137:2; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIGEN-ANTIBODY REACTIONS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CELL CONSTITUENTS
CELL MEMBRANES
ESTERS
FUNCTIONS
GLUCOPROTEINS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
KINETICS
LEUKOCYTES
MATERIALS
MEMBRANES
METABOLISM
NEUTROPHILS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PATIENTS
PEROXIDES
PHORBOL ESTERS
PROTEINS
RADICALS
REACTION KINETICS
SACCHARIDES
SUPEROXIDE RADICALS