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Stimulation of neutrophils by tumor necrosis factor

Journal Article · · J. Immunol.; (United States)
OSTI ID:5649587
; ; ; ; ; ;

Human recombinant tumor necrosis factor (TNF) was shown to be a weak direct stimulus of the neutrophil respiratory burst and degranulation. The stimulation, as measured by iodination, H/sub 2/O/sub 2/ production, and lysozyme release, was considerably increased by the presence of unopsonized zymosan in the reaction mixture, an effect which was associated with the increased ingestion of the zymosan. TNF does not act as an opsonin but, rather, reacts with the neutrophil to increase its phagocytic activity. TNF-dependent phagocytosis, as measured indirectly by iodination, is inhibited by monoclonal antibodies (Mab) 60.1 and 60.3, which recognize different epitopes on the C3bi receptor/adherence-promoting surface glycoprotein of neutrophils. Other neutrophil stimulants, namely N-formyl-methionyl-leucyl-phenylalanine, the Ca2+ ionophore A23187, and phorbol myristic acetate, also increase iodination in the presence of zymosan; as with TNF, the effect of these stimulants is inhibited by Mab 60.1 and 60.3, whereas, in contrast to that of TNF, their stimulation of iodination is unaffected by an Mab directed against TNF. TNF may be a natural stimulant of neutrophils which promotes adherence to endothelial cells and to particles, leading to increased phagocytosis, respiratory burst activity, and degranulation.

Research Organization:
Univ. of Washington, Seattle
OSTI ID:
5649587
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 11; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

550901 -- Pathology-- Tracer Techniques
560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARCINOGENS
DISEASES
ENZYMES
ESTERS
GLUCOPROTEINS
GLYCOSYL HYDROLASES
HEMIACETAL DEHYDROGENASES
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROLASES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LACTATE DEHYDROGENASE
LEUKOCYTES
LYSOZYME
MATERIALS
MONOCLONAL ANTIBODIES
NECROSIS
NEOPLASMS
NEUTROPHILS
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PEROXIDES
PHAGOCYTOSIS
PHENYLALANINE
PHORBOL ESTERS
PROTEINS
SACCHARIDES
TRACER TECHNIQUES
ZYMOSAN