Accumulation of sup 111 In-neutrophils in rabbit skin in allergic and non-allergic inflammatory reactions in vivo. Inhibition by neutrophil pretreatment in vitro with a monoclonal antibody recognizing the CD18 antigen

Journal Article · Mon May 01 04:00:00 EDT 1989 · Journal of Immunology; (USA)

OSTI ID:5514239

Nourshargh, S; Rampart, M; Hellewell, P G; Jose, P J; Harlan, J M; Edwards, A J; Williams, T J ^[1]

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1. MRC Clinical Research Centre, Harrow, Middlesex (England)

The mAb 60.3 recognizes the neutrophil CD18 Ag. We have investigated the effect of in vitro pretreatment of radiolabeled neutrophils with mAb 60.3 on their accumulation in vivo. Further, we have compared the in vivo effects of mAb 60.3 with its effects on neutrophil adherence in vitro. Neutrophil accumulation in vivo was measured in response to: (1) exogenous mediators FMLP, C5a des Arg, LTB4 and IL-1; (2) endogenous mediators generated in a non-allergic inflammatory reaction induced by zymosan; and (3) endogenous mediators generated in two allergic inflammatory reactions, a passive cutaneous anaphylactic reaction and a reversed passive Arthus reaction in rabbit skin. Pretreatment of neutrophils with mAb 60.3 inhibited their accumulation in all the responses. The results demonstrate that there is a common mechanism mediating neutrophil accumulation in these inflammatory reactions. Neutrophils pretreated with mAb 60.3 were also unresponsive to chemoattractants in in vitro adherence assays. However, the antibody-treated neutrophils responded normally to FMLP and C5a with respect to granular enzyme release. These results suggest that the basal expression of CD18 Ag is important for the adherence of neutrophils to microvascular endothelial cells stimulated by the local generation, or administration, of chemical mediators in vivo. Despite the fact that mediators such as FMLP can increase CD18 expression in vitro, it appears more likely that such mediators act in vivo by inducing a conformational change in the basally expressed neutrophil adhesive molecules.

• OSTI ID: 5514239
• Journal Information: Journal of Immunology; (USA), Journal Name: Journal of Immunology; (USA) Vol. 142:9; ISSN 0022-1767; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ALLERGY
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLYCOPROTEINS
IMMUNE SYSTEM DISEASES
IMMUNOSUPPRESSIVE DRUGS
INDIUM 111
INDIUM ISOTOPES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PROTEINS
RABBITS
RADIOISOTOPES
SKIN
SPECIFICITY
SYMPTOMS
TRACER TECHNIQUES
VERTEBRATES