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Phosphorylation of CD18 in response to neutrophil stimulation

Conference · · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
OSTI ID:5374640
; ;  [1]
  1. Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, CT (United States)
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Leukocyte integrins containing the common {beta}-subunit (CD18) mediate the adhesion of leukocytes to endothelial cells. It has been shown that the CD18 is phosphorylated in response to the phorbol ester PMA and proposed that phosphorylation of CD18 triggers the enhanced avidity of leukocyte integrins. The purpose of this study was to determine if CD18 in human neutrophils is also phosphorylated in response to physiological stimuli, i.e. receptor mediated activation. After labeling freshly isolated human neutrophils with {sup 32}p it was found that CD18 was phosphorylated in response to PMA in a time dependent manner that corresponded to the rate of PMA induced homotypic aggregation. The receptor mediated stimuli fMLP, LTB{sub 4}, IL-8, and C5a were also effective at initiating rapid CD18 dependent homotypic aggregation of neutrophils. However, in none of the receptor mediated responses was the level of CD18 phosphorylation increased at any time from the addition of stimuli to the peak of homotypic aggregation. Though not conclusive, this study suggests that in human neutrophils, activation of the leukocyte integrins by PMA may involve signaling pathways distinct from those involved in activation through receptor mediated stimulation.

OSTI ID:
5374640
Report Number(s):
CONF-9104107--
Journal Information:
FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN 0892-6638; ISSN FAJOE
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADHESION
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARCINOGENS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENDOTHELIUM
ESTERS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MAN
MATERIALS
METABOLISM
NEUTROPHILS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PRIMATES
PROTEINS
RADIOISOTOPES
TISSUES
TRACER TECHNIQUES
VERTEBRATES