Phosphorylation of cytosolic proteins by resting and activated human neutrophils
A study was conducted on the phosphorylation of proteins in the neutrophil cytosol in response to phorbol myristate acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). Autoradiography of gel electrophoretograms prepared from neutrophils incubated with /sup 32/P in the presence and absence of the activators showed nine proteins whose state of phosphorylation was affected by neutrophil activation. /sup 32/P was gained by eight of these proteins and was lost by the ninth. For all but one of these proteins, the change in the extent of labeling appeared to reach completion by one to two minutes. It was possible to quantitate the changes in /sup 32/P content of three of the nine proteins. One of these was the 20-kD protein that lost label when the neutrophils were activated. Quantitation showed that over half the /sup 32/P present in this protein in the resting state was gone within 0.2 minutes after activation. The other two were proteins weighing 11 and 69 kD. The phosphorylation characteristics of these two proteins differed, depending on whether activation had been carried out with PMA or fMLP. These differences in protein phosphorylation support other evidence suggesting that PMA and fMLP do not activate neutrophils by identical biochemical pathways.
- Research Organization:
- Tufts-New England Medical Center, Boston, MA
- OSTI ID:
- 5982164
- Journal Information:
- Blood; (United States), Journal Name: Blood; (United States) Vol. 64:4; ISSN BLOOA
- Country of Publication:
- United States
- Language:
- English
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550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETATES
AMINO ACIDS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
CYTOPLASM
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MATERIALS
NEUTROPHILS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENYLALANINE
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PROTEINS
RADIOISOTOPES