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Free sphingosine in control and stimulated human neutrophils

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6150476
; ; ; ; ;

Exogenous long-chain (sphingoid) bases are potent inhibitors of protein kinase C in vitro and the oxidative burst of human neutrophils. To assess their involvement in neutrophil function, high-performance liquid chromatography was used to quantitate free long-chain bases in neutrophils incubated with and without N-formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol 12-myristate 13-acetate (PMA). Sphingosine was present in freshly isolated neutrophils at 6.1 +/- 1.1 pmol/10W cells. During incubation at 37, the level increased several fold; further increases were obtained with fMLP whereas PMA decreased free sphingosine. This sphingosine was apparently derived from hydrolysis of complex sphingolipids. Since endogenous levels are comparable to those of diacylglycerols in neutrophils and, hence, within the range expected to affect the activity of protein kinase C, long-chain bases may serve a role in modulating neutrophil functions related to the action of this enzyme.

Research Organization:
Emory Univ., Atlanta, GA
OSTI ID:
6150476
Report Number(s):
CONF-870644-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
CHROMATOGRAPHY
ENZYME INHIBITORS
ENZYMES
ESTERS
FUNCTIONS
KINETICS
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENYLALANINE
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PRIMATES
REACTION KINETICS
SEPARATION PROCESSES
TRANSFERASES
VERTEBRATES