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Sources of variation in the mutagenic potency of complex chemical mixtures based on the salmonella/microsome assay

Technical Report ·
OSTI ID:5441157
; ; ;
Twenty laboratories worldwide participated in a collaborative trial sponsored by the International Program on Chemical Safety on the mutagenicity of complex mixtures as expressed in the Salmonella/microsome assay. The U.S. National Institute of Standards and Technology provided homogeneous reference samples of urban air and diesel particles and a coal tar solution to each participating laboratory, along with samples of benzo(a)pyrene and 1-nitropyrene which served as positive controls. Mutagenic potency was characterized by the slope of the initial linear component of the dose response curve. Analysis of variance revealed significant interlaboratory variation in mutagenic potency, which accounted for 57-96% of the total variance on a logarithmic scale, depending on the sample, strain and activation conditions. No significant differences were noted in the average potency reported for air and diesel particles between laboratories using soxhlet extracts and those using sonication, although there was larger interlaboratory variation for the soxhlet method.
Research Organization:
Environmental Protection Agency, Research Triangle Park, NC (United States). Health Effects Research Lab.
OSTI ID:
5441157
Report Number(s):
PB-92-150713/XAB; EPA--600/J-92/057
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
AROMATICS
BACTERIA
BENZOPYRENE
CELL CONSTITUENTS
COAL TAR
CONDENSED AROMATICS
DIESEL FUELS
DISPERSIONS
DOSE-RESPONSE RELATIONSHIPS
ENERGY SOURCES
FOSSIL FUELS
FUELS
GAS OILS
HYDROCARBONS
INTERLABORATORY COMPARISONS
LIQUID FUELS
MICROORGANISMS
MICROSOMES
MIXTURES
MUTAGEN SCREENING
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OTHER ORGANIC COMPOUNDS
PARTICLES
PARTICULATES
PETROLEUM
PETROLEUM FRACTIONS
PETROLEUM PRODUCTS
POLLUTION
RIBOSOMES
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
TAR