Petroleum distillates suppress in vitro metabolic activation: higher (S-9) required in the Salmonella/microsome mutagenicity assay
Journal Article
·
· Environ. Mutagen.; (United States)
To determine if standard conditions used in the Salmonella/mammalian microsome mutagenicity assay could reliably screen complex petroleum samples, two high-boiling (700-1070/sup 0/F) distillates and their separated aromatic fractions were tested. The initial mutagenic activities were inconsistent with the sample's known polyaromatic hydrocarbon (PAH) contents and observed potencies in a dermal carcinogenesis bioassay. A significant mutagenic response was observed only at S-9 concentrations 5 to 10 times higher than those used in the standard assay, supporting the use of elevated levels of S-9 in the Salmonella/microsome assay to assess the carcinogenic potential of petroleum-derived materials. All four samples masked the expected mutagenic activity of added PAHs (benzo(a)pyrene and perylene). Data suggested that petroleum distillates suppress the functional efficacy of the S-9; possible mechanisms are discussed.
- Research Organization:
- Chevron Environmental Health Center, Inc., Richmond, CA
- OSTI ID:
- 6240502
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 7:3; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BODY
CELL CONSTITUENTS
DISTILLATES
ENERGY SOURCES
FOSSIL FUELS
FUELS
HYDROCARBONS
INHIBITION
METABOLIC ACTIVATION
MICROORGANISMS
MICROSOMES
MUTAGEN SCREENING
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PETROLEUM
PETROLEUM DISTILLATES
PETROLEUM FRACTIONS
POLYCYCLIC AROMATIC HYDROCARBONS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SKIN
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BODY
CELL CONSTITUENTS
DISTILLATES
ENERGY SOURCES
FOSSIL FUELS
FUELS
HYDROCARBONS
INHIBITION
METABOLIC ACTIVATION
MICROORGANISMS
MICROSOMES
MUTAGEN SCREENING
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PETROLEUM
PETROLEUM DISTILLATES
PETROLEUM FRACTIONS
POLYCYCLIC AROMATIC HYDROCARBONS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SKIN