Mutagenicity of CTC No. 11 in the Ames Salmonella/Microsomal Assay

Technical Report · Wed Jul 15 04:00:00 EDT 1992

DOI:https://doi.org/10.2172/6713532· OSTI ID:6713532

NIOSH has studied the mutagenicity of seven mild gasification product samples using the Ames Salmonella/microsomal assay. The Ames assay is widely used as a short-term test for the detection of possible genotoxic agents and potential carcinogens. Bacterial tester strains used in the Ames assay contain specific mutations (frameshift or base pair substitution) the amino acid histidine. The assay was performed on CTC No. 11, a mild gasification product with a liquid/tar consistency, using a DMSO and Tween 80. CTC No. 11 displayed significant mutagenic activity in all conditions tested. The high response was noted on TA98with microsomal activation. Although both solvents allowed a strong response to be evident, the mutagenic activity was higher when DMSO was the solvent. Significant response under these conditions indicates thepresence of potent, indirect-acting, frameshift mutagens. Moderate significant mutagenic activity was also noted on TA98 without microsomal activation indicating the presence of a direct-acting frameshift mutagen. In this case, the response was slightly higher when Tween 80 was used the solvent. Mutagenic activity on TA100, which indicates base-pair substitutions, was moderate with microsomal activation and weak without.

• Research Organization: National Inst. for Occupational Safety and Health, Morgantown, WV (United States). Appalachian Lab. for Occupational Safety and Health
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AI21-89MC26018
• OSTI ID: 6713532
• Report Number(s): DOE/MC/26018-3163; ON: DE93006662
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
BACTERIA
COAL GASIFICATION
COAL LIQUIDS
COAL TAR
DMSO
DOCUMENT TYPES
FLUIDS
GASIFICATION
LIQUIDS
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OTHER ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC AMINES
PROGRESS REPORT
SALMONELLA
SCREENING
SULFOXIDES
TAR
THERMOCHEMICAL PROCESSES