Results of the IPCS collaborative study on complex mixtures

Technical Report · Wed Jan 01 04:00:00 EST 1992

OSTI ID:5307301

Claxton, L D; Creason, J; Leroux, B; Agurell, E; Bagley, S

The International Program on Chemical Safety (IPCS) sponsored a collaborative study to examine the intra- and interlaboratory variation associated with the preparation and bioassay of complex chemical mixtures. The mixtures selected were National Institute of Standards and Technology (NIST) Standard Reference Materials (SRM). Twenty laboratories worldwide participated in the study. The participating laboratories extracted the organic portion of two particulate samples--an air particulate sample and a diesel particulate sample--and bioassayed the extracts. The laboratories simultaneously bioassayed a NIST-prepared extract of coal tar and two control compounds (benzo(a)pyrene, and 1-nitropyrene). The bioassay method used was the Salmonella/mammalian microsome plate-incorporation test using strains TA98 and TA100. Study design also allowed for a comparison of sonication and Soxhlet extraction techniques. The particulates samples were mutagenic in both strains with and without activation in all twenty laboratories. The paper provides a complete summary of the data collected during the collaborative study.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (United States). Health Effects Research Lab.
• OSTI ID: 5307301
• Report Number(s): PB-92-150721/XAB; EPA--600/J-92/058
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
AROMATICS
BACTERIA
BENZOPYRENE
BIOASSAY
COAL TAR
CONDENSED AROMATICS
DIESEL FUELS
DISPERSIONS
ENERGY SOURCES
FOSSIL FUELS
FUELS
GAS OILS
HYDROCARBONS
INTERLABORATORY COMPARISONS
LIQUID FUELS
MICROORGANISMS
MIXTURES
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OTHER ORGANIC COMPOUNDS
PARTICLES
PARTICULATES
PETROLEUM
PETROLEUM FRACTIONS
PETROLEUM PRODUCTS
POLLUTION
SALMONELLA
SALMONELLA TYPHIMURIUM
TAR