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Comparison of mutagenicity and induction of sister chromatid exchange in Chinese hamster cells exposed to hematoporphyrin derivative photoradiation, ionizing radiation, or ultraviolet radiation

Journal Article · · Cancer Res.; (United States)
OSTI ID:5440269
; ; ;
Cell culture studies have been performed to compare the mutagenic potential and the induction of sister chromatid exchanges for hematoporphyrin derivative photoradiation, ionizing radiation, and UV radiation. The mutation frequency in Chinese hamster ovary cells at the hypoxanthine-guanine phosphoribosyltransferase locus was measured using resistance to 6-thioguanine. Phenotypic expression time prior to mutation selection was also examined. Treatment with either X-rays or UV was effective in producing mutants resistant to 6-thioguanine, but treatment with hematoporphyrin derivative photoradiation (at comparable toxicity levels) did not induce any mutagenic activity above background levels. The hematoporphyrin derivative incubation and photosensitization conditions used in this study did induce sister chromatid exchanges at frequencies comparable to those induced by X-rays but at lower frequencies than for UV treatments.
Research Organization:
Clayton Center for Ocular Oncology, Childrens Hospital of Los Angeles, California
OSTI ID:
5440269
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 43:6; ISSN CNREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
CARBOXYLIC ACIDS
CHEMISTRY
CHO CELLS
CHROMOSOMAL ABERRATIONS
ELECTROMAGNETIC RADIATION
HEMATOPORPHYRINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
MUTAGENESIS
MUTAGENS
MUTATION FREQUENCY
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMISTRY
PIGMENTS
PORPHYRINS
RADIATIONS
RADIOINDUCTION
SISTER CHROMATID EXCHANGES
ULTRAVIOLET RADIATION