Elastatinal and leupeptin: effects on u. v. -induced mutation and sister-chromatid exchanges in Chinese hamster cells
Journal Article
·
· Carcinogenesis (N.Y.); (United States)
Microbial protease inhibitors elastatinal and leupeptin were tested for cytotoxicity and for effects on spontaneous and u.v.-induced 6-thioguanine-resistant (6TGr) mutation and sister-chromatid exchange (SCE) in V79 Chinese hamster cells. Continuous treatment with elastatinal exhibited marked cytotoxicity, while leupeptin was almost non-cytotoxic. Elastatinal rapidly induced cytotoxic effects as a function of its concentration and time of exposure. Near maximum cytotoxicity was reached after exposure of 6-8 h and this was partially abolished by the presence of 2.5 micrograms cycloheximide per ml. Concentrations of either protease inhibitor which gave 60-80% survival had no appreciable effects on u.v. survival and frequencies of spontaneous and u.v.-induced 6TGr mutation and SCE. However, reconstruction experiments revealed that pretreatments of 6TGr and 6TGs (wild-type) cells with these inhibitors for 6 days tended to block metabolic co-operation in their co-cultures. Thus, elastatinal and leupeptin are neither clastogenic mutagenic by themselves, and do not alter mutation fixation and expression.
- OSTI ID:
- 5440659
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 2:4; ISSN CRNGD
- Country of Publication:
- United States
- Language:
- English
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Conference
·
Wed Aug 01 00:00:00 EDT 1979
· Radiat. Res.; (United States)
·
OSTI ID:5639189
Comparison of 6-thioguanine-resistant mutation and sister chromatid exchanges in Chinese hamster V79 cells with forty chemical and physical agents
Journal Article
·
Wed Aug 01 00:00:00 EDT 1984
· Cancer Res.; (United States)
·
OSTI ID:6304795
Comparison of mutagenicity and induction of sister chromatid exchange in Chinese hamster cells exposed to hematoporphyrin derivative photoradiation, ionizing radiation, or ultraviolet radiation
Journal Article
·
Wed Jun 01 00:00:00 EDT 1983
· Cancer Res.; (United States)
·
OSTI ID:5440269
Related Subjects
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
CELL CULTURES
CHROMOSOMAL ABERRATIONS
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
HAMSTERS
MAMMALS
MUTAGENESIS
MUTATIONS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RODENTS
SISTER CHROMATID EXCHANGES
TOXICITY
ULTRAVIOLET RADIATION
VERTEBRATES
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
CELL CULTURES
CHROMOSOMAL ABERRATIONS
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
HAMSTERS
MAMMALS
MUTAGENESIS
MUTATIONS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RODENTS
SISTER CHROMATID EXCHANGES
TOXICITY
ULTRAVIOLET RADIATION
VERTEBRATES