The effects of radioiodination and fluorescent labelling on albumin

Journal Article · Thu Jan 01 00:00:00 EST 1981 · Prep. Biochem.; (United States)

DOI:https://doi.org/10.1080/00327488108064237· OSTI ID:5405144

Crandall, R E; Janatova, J; Andrade, J D

The preparation and characterization of fluorescamine -, fluorescein isothiocyanate (FITC) -, and radioiodine-labelled bovine serum albumin is critically evaluated. Electrophoretic mobility and ion-exchange chromatography, together with measures of degree of conjugation and sulfhydryl content, are used to assess the changes due to conjugation. Fluorescamine labelling results in drastic changes in chromatographic behavior and electrophoretic mobility. FITC labelling also results in significant changes in chromatographic and electrophoretic properties. Radioiodination leads to minor changes in chromatographic properties and oxydation of sulfhydryl groups, with little or no change in electrophoretic properties. All three labels have some degree of lability and show increased levels of free label with time, even after extensive initial purification. It is concluded that the two fluorescent labels and possibly the radioiodine labelling method used here are unsuitable for certain studies of BSA, such as its adsorption at solid-liquid interfaces.

• OSTI ID: 5405144
• Journal Information: Prep. Biochem.; (United States), Vol. 11:2
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
CHEMICAL RADIATION EFFECTS
ISOTHIOCYANATES
LABELLED COMPOUNDS
CHEMICAL PROPERTIES
BLOOD SERUM
CATTLE
ELECTROPHORESIS
FLUORESCEIN
IODINE ISOTOPES
ION EXCHANGE CHROMATOGRAPHY
ION MOBILITY
MEASURING METHODS
ANIMALS
AROMATICS
CARBOXYLIC ACIDS
CHEMISTRY
CHROMATOGRAPHY
DOMESTIC ANIMALS
DYES
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPES
MAMMALS
MOBILITY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARTICLE MOBILITY
PHENOLS
POLYPHENOLS
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RUMINANTS
SEPARATION PROCESSES
VERTEBRATES
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