The effects of radioiodination and fluorescent labelling on albumin

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Prep. Biochem.; (United States)

DOI:https://doi.org/10.1080/00327488108064237· OSTI ID:5405144

Crandall, R E; Janatova, J; Andrade, J D

The preparation and characterization of fluorescamine -, fluorescein isothiocyanate (FITC) -, and radioiodine-labelled bovine serum albumin is critically evaluated. Electrophoretic mobility and ion-exchange chromatography, together with measures of degree of conjugation and sulfhydryl content, are used to assess the changes due to conjugation. Fluorescamine labelling results in drastic changes in chromatographic behavior and electrophoretic mobility. FITC labelling also results in significant changes in chromatographic and electrophoretic properties. Radioiodination leads to minor changes in chromatographic properties and oxydation of sulfhydryl groups, with little or no change in electrophoretic properties. All three labels have some degree of lability and show increased levels of free label with time, even after extensive initial purification. It is concluded that the two fluorescent labels and possibly the radioiodine labelling method used here are unsuitable for certain studies of BSA, such as its adsorption at solid-liquid interfaces.

• OSTI ID: 5405144
• Journal Information: Prep. Biochem.; (United States), Journal Name: Prep. Biochem.; (United States) Vol. 11:2; ISSN PRBCB
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
ANIMALS
AROMATICS
BLOOD SERUM
CARBOXYLIC ACIDS
CATTLE
CHEMICAL PROPERTIES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CHROMATOGRAPHY
DOMESTIC ANIMALS
DYES
ELECTROPHORESIS
FLUORESCEIN
HYDROXY ACIDS
HYDROXY COMPOUNDS
IODINE ISOTOPES
ION EXCHANGE CHROMATOGRAPHY
ION MOBILITY
ISOTHIOCYANATES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEASURING METHODS
MOBILITY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARTICLE MOBILITY
PHENOLS
POLYPHENOLS
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RUMINANTS
SEPARATION PROCESSES
VERTEBRATES