Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives
- Kyung Hee University, Department of Anatomy and Neurobiology, College of Medicine (Korea, Republic of)
- Pohang University of Science and Technology (POSTECH), Department of Chemistry (Korea, Republic of)
- Kyung Hee University, Department of Biomedical Science, Graduate School (Korea, Republic of)
- Korea University, Department of Chemistry (Korea, Republic of)
8-Amino-BODIPY (boron-dipyrromethane) dyes show bright blue fluorescence. Disclosed here are synthesis and characterization of the photophysical properties of a series of functionalized 8-Amino-BODIPY (BP1–4) for protein labeling. The compact structure and solvent-insensitive absorption property of the dye are desirable features for protein labeling. For the model protein, bovine serum albumin (BSA), the labeling proceeds under mild condition via amide bond formation or thiol-ene conjugation with maintaining the bright blue fluorescence. The chromatography and mass spectroscopy analysis clearly support the labeling of the BODIPY dye on the BSA. The protein labeling with blue-emitting BODIPY would be applicable for studying protein dynamics and fluorescence resonance energy transfer (FRET) with intrinsic biomolecules.
- OSTI ID:
- 22795723
- Journal Information:
- Journal of Fluorescence (Online), Journal Name: Journal of Fluorescence (Online) Journal Issue: 6 Vol. 27; ISSN 1573-4994
- Country of Publication:
- United States
- Language:
- English
Similar Records
Pulsed laser fluorometry for environmental monitoring
Nanoparticle-based energy transfer for rapid and simple detection of protein glycosylation