Influence of radioiodination on the adsorption of IgG and serum albumin to polystyrene

Journal Article · Sat Nov 01 04:00:00 EST 1986 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(86)90569-5· OSTI ID:5871100

Walsh, J; Gosling, J P

The adsorption of radioiodinated rabbit IgG and bovine serum albumin (BSA) to polystyrene tubes was investigated. Adsorption isotherms where the proportion of the protein bound was relatively constant over a range of intermediate protein concentrations, and where the proportion bound was protein dependent, were obtained. To investigate the effects of radioiodination, proteins labelled to give a wide range of substitution ratios (0.03 to 3.7 /sup 125/I/ protein molecule) were employed. While labeling did not appear to affect BSA adsorption, the kinetics of IgG binding were altered in a number of ways. The proportion bound in the concentration independent region was decreased even at substitution ratios less than or equal to 0.2. In addition, while all preparations of iodinated BSA, and IgG preparations with less than or equal to 1.6 /sup 125/I/IgG, gave bimodal adsorption isotherms, with more heavily labeled IgG (less than or equal to 2.5 /sup 125/I/IgG) the apparent high affinity binding to the plastic surface was abolished. These results indicate that radioiodination substantially alters the kinetics of the binding of IgG to polystyrene. In addition, the results obtained are discussed with respect to previous relevant and often apparently contradictory findings.

• Research Organization: Univ. College, Galway, Ireland
• OSTI ID: 5871100
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 158:2; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

560160* -- Radionuclide Effects
Kinetics
& Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADSORPTION
ADSORPTION ISOTHERMS
ALBUMINS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BLOOD SERUM
CATTLE
CHEMICAL REACTIONS
CONFIGURATION INTERACTION
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HALOGENATION
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTHERMS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLYMERS
POLYOLEFINS
POLYSTYRENE
POLYVINYLS
PROTEINS
RABBITS
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
SORPTION
SYNTHETIC MATERIALS
VERTEBRATES