Effects of argon laser radiation on aortic endothelial cells: Early membrane changes and proliferative response

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Journal of Surgical Research; (USA)

DOI:https://doi.org/10.1016/0022-4804(89)90024-3· OSTI ID:5399834

Franceschi, D; Graham, D; Alexander, J J; Koehler, K ^[1]

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1. Case Western Reserve Univ., OH (USA)

Membrane fluidity, transmembrane signaling responses, and proliferative characteristics of endothelial cells were studied to characterize biochemical and molecular changes after treatment with argon laser energy. Bovine aortic endothelial cells grown in monolayers were irradiated at 50, 100, and 200 J with an argon laser (wavelength, 488 and 514 nm). Proliferation, assayed by ({sup }3H)thymidine incorporation, was measured daily for 6 days. An initial lag phase was observed for irradiated cells when compared to nonirradiated controls (P less than 0.03), with eventual recovery by the third day. Membrane fluidity, determined by fluorescence anisotropy, was measured 1 hr after irradiation. A decrease in static rotational motion of 1,6-diphenyl-1,3,5-hexatriene (DPH) was noted in irradiated versus nonirradiated cells indicating a decrease in membrane fluidity (P less than 0.02). Dynamic studies of intracellular calcium and pH flux utilizing fluorescent probes demonstrated a preserved response to mitogenic stimulation. An increase in intracellular Ca2+ with a concomitant alkalinization of the intracellular milieu was observed in irradiated and non-irradiated cells in response to stimulation with endothelial cell growth factor (ECGF). These responses resemble those characterized for other mitogens. Argon laser energy applied to aortic endothelial cells decreases membrane fluidity early after irradiation. These alterations probably cause the initial lag observed in their proliferative response; however, the capacity to respond to exogenous mitogenic stimulation is maintained.

• OSTI ID: 5399834
• Journal Information: Journal of Surgical Research; (USA), Journal Name: Journal of Surgical Research; (USA) Vol. 46:6; ISSN 0022-4804; ISSN JSGRA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METALS
ANIMAL TISSUES
ANIMALS
AORTA
ARGON
ARTERIES
AZINES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CALCIUM
CARDIOVASCULAR SYSTEM
CATTLE
CELL CONSTITUENTS
CELL CULTURES
CELL DIVISION
CELL MEMBRANES
CELL PROLIFERATION
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ELEMENTS
ENDOTHELIUM
FLUIDS
GASES
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LASER RADIATION
LASERS
MAMMALS
MEMBRANES
METALS
MITOGENS
NONMETALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RADIATIONS
RARE GASES
RIBOSIDES
RUMINANTS
SENSITIVITY
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES