Stimulation of radiation-impaired plasminogen activator release by phorbol ester in aortic endothelial cells

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Proceedings of the Society for Experimental Biology and Medicine; (USA)

DOI:https://doi.org/10.3181/00379727-195-43137· OSTI ID:6267371

Raymond, J; Yoon, S C; Ts'ao, C H ^[1]

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1. Northwestern Univ. School of Medicine, Chicago, IL (USA)

Ionizing radiation has been reported to affect the fibrinolytic activity of exposed tissue. With cultured bovine aortic endothelial cells, radiation suppresses the release of plasminogen activator to the conditioned media, with a concomitant increase in intracellular plasminogen activator. Thus study was undertaken to determine whether radiation-impaired plasminogen activator release can be modified by phorbol ester. We exposed cultured bovine aortic endothelial cells to a sterilizing dose of 10 Gy of gamma-rays and found the treatment led to cell injury, as evidenced by an increased release of prelabeled chromium, and to a reduction of plasminogen activator in the conditioned media with elevated intracellular plasminogen activator in irradiated cells. Phorbol ester enhanced plasminogen activator activity in both sham-irradiated and irradiated endothelial cells. It was interesting to note that the increased plasminogen activator in phorbol ester-stimulated sham-irradiated cells was largely retained inside the cell, while it was released to the conditioned media in irradiated cells. Apparently, altered plasminogen activator activity of radiation-sterilized endothelial cells can be modified by exogenous stimuli.

• OSTI ID: 6267371
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine; (USA), Journal Name: Proceedings of the Society for Experimental Biology and Medicine; (USA) Vol. 195:2; ISSN PSEBA; ISSN 0037-9727
• Country of Publication: United States
• Language: English

Related Subjects

560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD VESSELS
BODY
CARCINOGENS
CARDIOVASCULAR SYSTEM
CATTLE
DNA
DOMESTIC ANIMALS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOTHELIUM
ESTERS
FIBRINOLYTIC AGENTS
GAMMA RADIATION
HEMATOLOGIC AGENTS
IN VITRO
IONIZING RADIATIONS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHORBOL ESTERS
PLASMINOGEN
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RUMINANTS
SECRETION
TISSUES
VERTEBRATES