Acute radiation effects on the content and release of plasminogen activator activity in cultured aortic endothelial cells

Journal Article · Fri Feb 01 04:00:00 EST 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576403· OSTI ID:5763832

Ts'ao, C H; Ward, W F

Confluent monolayers from three lines of bovine aortic endothelial cells were exposed to a single dose of 10 Gy of /sup 60/Co ..gamma.. rays. Seventy-two hours later, the morphology of the irradiated and sham-irradiated monolayers was examined, and cellular DNA and protein contents were determined. In addition, the release of plasminogen activator (PA) activity into the culture media and PA activity in the cell lysates were assayed. DNA and protein contents in the irradiated monolayers were reduced to 43-50% and 72-95% of the control levels, respectively. These data indicate that radiation induced cell loss (detachment and/or lysis) from the monolayer, with hypertrophy of surviving (attached) cells to preserve the continuity of the monolayer surface. Total PA activity (lysate plus medium) in the irradiated dishes was reduced to 50-75% of the control level. However, when endothelial PA activity was expressed on the basis of DNA content, the irradiated monolayers from two of the three cell lines contained significantly more PA activity than did sham-irradiated monolayers. These data suggest that fibrinolytic defects observed in irradiated tissues in situ may be attributable at least in part to a radiation-induced inhibition of PA release by vascular endothelial cells.

• Research Organization: Northwestern Univ. Medical School, Chicago, IL
• OSTI ID: 5763832
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 101:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CATTLE
CELL CULTURES
CHEMISTRY
COBALT 60
COBALT ISOTOPES
COWS
DNA
DOMESTIC ANIMALS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOTHELIUM
ENZYME ACTIVITY
FIBRINOLYTIC AGENTS
FIBROSIS
GAMMA RADIATION
HEMATOLOGIC AGENTS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
MORPHOLOGICAL CHANGES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PLASMINOGEN
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RUMINANTS
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES