Enzymatic responses to radiation in cultured vascular endothelial and smooth muscle cells

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576384· OSTI ID:6539756

Rubin, D B; Drab, E A; Ward, W F; Smith, L J; Fowell, S M

Confluent monolayers of bovine aortic endothelial and smooth muscle cells were exposed to 0-5.0 Gy of /sup 60/Co ..gamma.. rays. From 0 to 72 hr after irradiation, the monolayer and culture medium were analyzed for cell (nuclei) number, DNA and protein content, the activities of angiotensin converting enzyme (ACE), lactate dehydrogenase (LDH), and superoxide dismutase (SOD), and LDH isoenzyme profile. Irradiated endothelial cells exhibited a time- and dose-dependent increase in cell detachment, decreased DNA and protein content and reduced ACE activity per attached cell, increased LDH and SOD activities per microgram of DNA, and increased LDH activity in the culture medium. The release of LDH activity, observed after 0.5 Gy, was the most sensitive endothelial response, and occurred independent of or preceding cell detachment. Vascular smooth muscle cells contained two or three times more SOD activity than did endothelial cells and exhibited no significant responses to 5.0 Gy.

• Research Organization: Northwestern Univ. Medical School, Chicago, IL
• OSTI ID: 6539756
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 99:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANGIOTENSIN
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARDIOVASCULAR AGENTS
CATTLE
CELL CONSTITUENTS
CELL NUCLEI
CHEMISTRY
COBALT 60
COBALT ISOTOPES
DATA
DNA
DOMESTIC ANIMALS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOTHELIUM
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
GAMMA RADIATION
GLOBULINS
HEMIACETAL DEHYDROGENASES
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOENZYMES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LACTATE DEHYDROGENASE
MAMMALS
MINUTES LIVING RADIOISOTOPES
MUSCLES
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RUMINANTS
SUPEROXIDE DISMUTASE
TISSUES
VASOCONSTRICTORS
VERTEBRATES
YEARS LIVING RADIOISOTOPES