Effect of dexamethasone on the cytotoxic and enzymatic response of cultured endothelial cells to radiation

Journal Article · Thu Aug 01 04:00:00 EDT 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576578· OSTI ID:5198444

Lam, T T; Rubin, D B; Drab, E A

Experiments were conducted to determine (1) whether glucocorticoids directly protected endothelial cells (EC) from radiation and (2) if angiotensin converting enzyme (ACE) activity, known to be increased by glucocorticoid, played a role in the EC response to radiation. Confluent monolayers of EC cultured from bovine aorta EC were treated with dexamethasone (10/sup -6/ M); after irradiation (5.0 Gy, /sup 60/Co ..gamma..) ACE and lactate dehydrogenase (LDH) activities, DNA and protein contents, and nuclei number were measured. Combined dexamethasone treatment and radiation increased cellular ACE activity at a time when neither agent alone had an effect (24-hr dexamethasone exposure before 5 Gy and assayed 24 hr after 5 Gy). This interaction between radiation and dexamethasone treatment suggests that the glucocorticoid modifies the cell's response to injury. Although this interaction does not ameliorate radiation cytotoxicity, maintenance of ACE levels in injured vessels by hormones may have physiological significance in the hemodynamics of irradiated tissues.

• Research Organization: Northwestern Univ. Medical School, Chicago, IL
• OSTI ID: 5198444
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 103:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ANGIOTENSIN
ANIMAL CELLS
ANIMAL TISSUES
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD VESSELS
BODY
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL NUCLEI
COBALT 60
COBALT ISOTOPES
CORTICOSTEROIDS
DEXAMETHASONE
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ENDOTHELIUM
ENZYME ACTIVITY
ENZYMES
GAMMA RADIATION
GLOBULINS
GLUCOCORTICOIDS
HEMIACETAL DEHYDROGENASES
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KETONES
LACTATE DEHYDROGENASE
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PREGNANES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
STEROID HORMONES
STEROIDS
TISSUES
TOXICITY
VASOCONSTRICTORS
YEARS LIVING RADIOISOTOPES