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Interaction of endothelial cell growth factor with heparin: characterization by receptor and antibody recognition

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occupancy by the ligand. Heparin interacts structurally with ECGF, potentiates the mitogenic activity of the polypeptide, restores the biological activity to inactivate ECGF, enhances the affinity of the ligand to cell surface receptors, and modifies antibody recognition of ECFG. These data suggest that the association between heparin and ECGF induces a conformational change in the polypeptide that increases or stabilizes that biological activity of the mitogen. ECGF was radiolabeled with iodine-125.
Research Organization:
Institute of Biological Sciences, Palo Alto, CA
OSTI ID:
6300448
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 82:18; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CONFIGURATION INTERACTION
CONFORMATIONAL CHANGES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
FIBROBLASTS
HEMATOLOGIC AGENTS
HEPARIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MAN
MEMBRANES
MICE
MONOCLONAL ANTIBODIES
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
POLYPEPTIDES
POLYSACCHARIDES
PRIMATES
PROTEINS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RADIORECEPTOR ASSAY
RODENTS
SACCHARIDES
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
VERTEBRATES